plasmids 17217 17218 17219 Search Results


plasmids 17217 17218 17219 17220  (Addgene inc)
93
Addgene inc plasmids 17217 17218 17219 17220
Plasmids 17217 17218 17219 17220, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmids 17217 17218 17219 17220 - by Bioz Stars, 2026-09
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pmxs cymc  (Addgene inc)
93
Addgene inc pmxs cymc
Pmxs Cymc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+17217+17218+17219/pMXs-hSOX2+(Plasmid+%2317218)/pmc06588580-258-41-42
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klf4  (Addgene inc)
94
Addgene inc klf4
Fig. 1. Characterization of G15.AO hiPSC line. (A) G15.AO hiPSC line displays a typical round shape colony morphology with small, tightly packed cells (left panel). Alkaline phosphatase enzymatic activity (Right panel). (B) Endogenous pluripotency-associated markers NANOG, LIN28a, REX1, OCT4, SOX2, <t>KLF4,</t> and cMYC were confirmed by qPCR. MSU hiPSC line and pa- rental ADSCs (G15) were used as positive and negative controls, respectively. (C) Silencing of exogenous reprogramming factors was confirmed by qPCR after passage 15. (D) Expression of pluripotency-associated markers NANOG, OCT4, SOX2, and TRA1-81 at protein level by immunofluorescence. (E) Expression of pluripotency-associated markers TRA1-60, SSEA4, and SSEA3 by FACS analysis. The upper panel shows the staining using the corresponding irrelevant isotype-matched antibody. (F) In vitro differentiation study by EB formation. EBs were grown for 21 days and induction of genes representative of the three germ layers were analyzed by RT-PCR. Parental ADSCs (G15) were used as negative control. (G) In vivo differentiation test by teratoma formation assay. The pictures show hematoxylin/eosin staining (H&E) with representative tissues from the three germ layers. Pseudostratified epithelium similar to trachea (left), cartilage tissue (middle), and glandular tissues (right).
Klf4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+17217+17218+17219/pMXs-hc-MYC+(Plasmid+%2317220)/pm27345779-39-23-24
Average 94 stars, based on 1 article reviews
klf4 - by Bioz Stars, 2026-09
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retroviral vectors  (Addgene inc)
93
Addgene inc retroviral vectors
Fig. 1. Characterization of G15.AO hiPSC line. (A) G15.AO hiPSC line displays a typical round shape colony morphology with small, tightly packed cells (left panel). Alkaline phosphatase enzymatic activity (Right panel). (B) Endogenous pluripotency-associated markers NANOG, LIN28a, REX1, OCT4, SOX2, <t>KLF4,</t> and cMYC were confirmed by qPCR. MSU hiPSC line and pa- rental ADSCs (G15) were used as positive and negative controls, respectively. (C) Silencing of exogenous reprogramming factors was confirmed by qPCR after passage 15. (D) Expression of pluripotency-associated markers NANOG, OCT4, SOX2, and TRA1-81 at protein level by immunofluorescence. (E) Expression of pluripotency-associated markers TRA1-60, SSEA4, and SSEA3 by FACS analysis. The upper panel shows the staining using the corresponding irrelevant isotype-matched antibody. (F) In vitro differentiation study by EB formation. EBs were grown for 21 days and induction of genes representative of the three germ layers were analyzed by RT-PCR. Parental ADSCs (G15) were used as negative control. (G) In vivo differentiation test by teratoma formation assay. The pictures show hematoxylin/eosin staining (H&E) with representative tissues from the three germ layers. Pseudostratified epithelium similar to trachea (left), cartilage tissue (middle), and glandular tissues (right).
Retroviral Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+17217+17218+17219/pMXs-hKLF4+(Plasmid+%2317219)/pmc03841145-42-3-8
Average 93 stars, based on 1 article reviews
retroviral vectors - by Bioz Stars, 2026-09
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pcmv vsvg  (Addgene inc)
96
Addgene inc pcmv vsvg
Fig. 1. Characterization of G15.AO hiPSC line. (A) G15.AO hiPSC line displays a typical round shape colony morphology with small, tightly packed cells (left panel). Alkaline phosphatase enzymatic activity (Right panel). (B) Endogenous pluripotency-associated markers NANOG, LIN28a, REX1, OCT4, SOX2, <t>KLF4,</t> and cMYC were confirmed by qPCR. MSU hiPSC line and pa- rental ADSCs (G15) were used as positive and negative controls, respectively. (C) Silencing of exogenous reprogramming factors was confirmed by qPCR after passage 15. (D) Expression of pluripotency-associated markers NANOG, OCT4, SOX2, and TRA1-81 at protein level by immunofluorescence. (E) Expression of pluripotency-associated markers TRA1-60, SSEA4, and SSEA3 by FACS analysis. The upper panel shows the staining using the corresponding irrelevant isotype-matched antibody. (F) In vitro differentiation study by EB formation. EBs were grown for 21 days and induction of genes representative of the three germ layers were analyzed by RT-PCR. Parental ADSCs (G15) were used as negative control. (G) In vivo differentiation test by teratoma formation assay. The pictures show hematoxylin/eosin staining (H&E) with representative tissues from the three germ layers. Pseudostratified epithelium similar to trachea (left), cartilage tissue (middle), and glandular tissues (right).
Pcmv Vsvg, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+17217+17218+17219/pCMV-VSV-G+(Plasmid+%238454)/pm32950023-33-12-17
Average 96 stars, based on 1 article reviews
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pumvc  (Addgene inc)
95
Addgene inc pumvc
Fig. 1. Characterization of G15.AO hiPSC line. (A) G15.AO hiPSC line displays a typical round shape colony morphology with small, tightly packed cells (left panel). Alkaline phosphatase enzymatic activity (Right panel). (B) Endogenous pluripotency-associated markers NANOG, LIN28a, REX1, OCT4, SOX2, <t>KLF4,</t> and cMYC were confirmed by qPCR. MSU hiPSC line and pa- rental ADSCs (G15) were used as positive and negative controls, respectively. (C) Silencing of exogenous reprogramming factors was confirmed by qPCR after passage 15. (D) Expression of pluripotency-associated markers NANOG, OCT4, SOX2, and TRA1-81 at protein level by immunofluorescence. (E) Expression of pluripotency-associated markers TRA1-60, SSEA4, and SSEA3 by FACS analysis. The upper panel shows the staining using the corresponding irrelevant isotype-matched antibody. (F) In vitro differentiation study by EB formation. EBs were grown for 21 days and induction of genes representative of the three germ layers were analyzed by RT-PCR. Parental ADSCs (G15) were used as negative control. (G) In vivo differentiation test by teratoma formation assay. The pictures show hematoxylin/eosin staining (H&E) with representative tissues from the three germ layers. Pseudostratified epithelium similar to trachea (left), cartilage tissue (middle), and glandular tissues (right).
Pumvc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+17217+17218+17219/pUMVC+(Plasmid+%238449)/pm33275284-33-17-18
Average 95 stars, based on 1 article reviews
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fudeltagw rtta  (Addgene inc)
96
Addgene inc fudeltagw rtta
Fig. 1. Characterization of G15.AO hiPSC line. (A) G15.AO hiPSC line displays a typical round shape colony morphology with small, tightly packed cells (left panel). Alkaline phosphatase enzymatic activity (Right panel). (B) Endogenous pluripotency-associated markers NANOG, LIN28a, REX1, OCT4, SOX2, <t>KLF4,</t> and cMYC were confirmed by qPCR. MSU hiPSC line and pa- rental ADSCs (G15) were used as positive and negative controls, respectively. (C) Silencing of exogenous reprogramming factors was confirmed by qPCR after passage 15. (D) Expression of pluripotency-associated markers NANOG, OCT4, SOX2, and TRA1-81 at protein level by immunofluorescence. (E) Expression of pluripotency-associated markers TRA1-60, SSEA4, and SSEA3 by FACS analysis. The upper panel shows the staining using the corresponding irrelevant isotype-matched antibody. (F) In vitro differentiation study by EB formation. EBs were grown for 21 days and induction of genes representative of the three germ layers were analyzed by RT-PCR. Parental ADSCs (G15) were used as negative control. (G) In vivo differentiation test by teratoma formation assay. The pictures show hematoxylin/eosin staining (H&E) with representative tissues from the three germ layers. Pseudostratified epithelium similar to trachea (left), cartilage tissue (middle), and glandular tissues (right).
Fudeltagw Rtta, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+17217+17218+17219/FUdeltaGW-rtTA+(Plasmid+%2319780)/10__1074_slash_jbc__m112__384024-252-5-14
Average 96 stars, based on 1 article reviews
fudeltagw rtta - by Bioz Stars, 2026-09
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l myc  (Addgene inc)
92
Addgene inc l myc
Fig. 1. Characterization of G15.AO hiPSC line. (A) G15.AO hiPSC line displays a typical round shape colony morphology with small, tightly packed cells (left panel). Alkaline phosphatase enzymatic activity (Right panel). (B) Endogenous pluripotency-associated markers NANOG, LIN28a, REX1, OCT4, SOX2, <t>KLF4,</t> and cMYC were confirmed by qPCR. MSU hiPSC line and pa- rental ADSCs (G15) were used as positive and negative controls, respectively. (C) Silencing of exogenous reprogramming factors was confirmed by qPCR after passage 15. (D) Expression of pluripotency-associated markers NANOG, OCT4, SOX2, and TRA1-81 at protein level by immunofluorescence. (E) Expression of pluripotency-associated markers TRA1-60, SSEA4, and SSEA3 by FACS analysis. The upper panel shows the staining using the corresponding irrelevant isotype-matched antibody. (F) In vitro differentiation study by EB formation. EBs were grown for 21 days and induction of genes representative of the three germ layers were analyzed by RT-PCR. Parental ADSCs (G15) were used as negative control. (G) In vivo differentiation test by teratoma formation assay. The pictures show hematoxylin/eosin staining (H&E) with representative tissues from the three germ layers. Pseudostratified epithelium similar to trachea (left), cartilage tissue (middle), and glandular tissues (right).
L Myc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+17217+17218+17219/pMXs-Hu-L-Myc+(Plasmid+%2326022)/pm26668030-56-25-26
Average 92 stars, based on 1 article reviews
l myc - by Bioz Stars, 2026-09
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pmxs hlin28a  (Addgene inc)
92
Addgene inc pmxs hlin28a
Fig. 1. Characterization of G15.AO hiPSC line. (A) G15.AO hiPSC line displays a typical round shape colony morphology with small, tightly packed cells (left panel). Alkaline phosphatase enzymatic activity (Right panel). (B) Endogenous pluripotency-associated markers NANOG, LIN28a, REX1, OCT4, SOX2, <t>KLF4,</t> and cMYC were confirmed by qPCR. MSU hiPSC line and pa- rental ADSCs (G15) were used as positive and negative controls, respectively. (C) Silencing of exogenous reprogramming factors was confirmed by qPCR after passage 15. (D) Expression of pluripotency-associated markers NANOG, OCT4, SOX2, and TRA1-81 at protein level by immunofluorescence. (E) Expression of pluripotency-associated markers TRA1-60, SSEA4, and SSEA3 by FACS analysis. The upper panel shows the staining using the corresponding irrelevant isotype-matched antibody. (F) In vitro differentiation study by EB formation. EBs were grown for 21 days and induction of genes representative of the three germ layers were analyzed by RT-PCR. Parental ADSCs (G15) were used as negative control. (G) In vivo differentiation test by teratoma formation assay. The pictures show hematoxylin/eosin staining (H&E) with representative tissues from the three germ layers. Pseudostratified epithelium similar to trachea (left), cartilage tissue (middle), and glandular tissues (right).
Pmxs Hlin28a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+17217+17218+17219/pMXs-hLIN28A+(Plasmid+%2347902)/pm29034889-49-19-5
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pmxs plasmids  (Addgene inc)
93
Addgene inc pmxs plasmids
Fig. 1. Characterization of G15.AO hiPSC line. (A) G15.AO hiPSC line displays a typical round shape colony morphology with small, tightly packed cells (left panel). Alkaline phosphatase enzymatic activity (Right panel). (B) Endogenous pluripotency-associated markers NANOG, LIN28a, REX1, OCT4, SOX2, <t>KLF4,</t> and cMYC were confirmed by qPCR. MSU hiPSC line and pa- rental ADSCs (G15) were used as positive and negative controls, respectively. (C) Silencing of exogenous reprogramming factors was confirmed by qPCR after passage 15. (D) Expression of pluripotency-associated markers NANOG, OCT4, SOX2, and TRA1-81 at protein level by immunofluorescence. (E) Expression of pluripotency-associated markers TRA1-60, SSEA4, and SSEA3 by FACS analysis. The upper panel shows the staining using the corresponding irrelevant isotype-matched antibody. (F) In vitro differentiation study by EB formation. EBs were grown for 21 days and induction of genes representative of the three germ layers were analyzed by RT-PCR. Parental ADSCs (G15) were used as negative control. (G) In vivo differentiation test by teratoma formation assay. The pictures show hematoxylin/eosin staining (H&E) with representative tissues from the three germ layers. Pseudostratified epithelium similar to trachea (left), cartilage tissue (middle), and glandular tissues (right).
Pmxs Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+17217+17218+17219/pMXs-Klf4+(Plasmid+%2313370)/pm27939217-280-2-18
Average 93 stars, based on 1 article reviews
pmxs plasmids - by Bioz Stars, 2026-09
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pmxs gw  (Addgene inc)
93
Addgene inc pmxs gw
Fig. 1. Characterization of G15.AO hiPSC line. (A) G15.AO hiPSC line displays a typical round shape colony morphology with small, tightly packed cells (left panel). Alkaline phosphatase enzymatic activity (Right panel). (B) Endogenous pluripotency-associated markers NANOG, LIN28a, REX1, OCT4, SOX2, <t>KLF4,</t> and cMYC were confirmed by qPCR. MSU hiPSC line and pa- rental ADSCs (G15) were used as positive and negative controls, respectively. (C) Silencing of exogenous reprogramming factors was confirmed by qPCR after passage 15. (D) Expression of pluripotency-associated markers NANOG, OCT4, SOX2, and TRA1-81 at protein level by immunofluorescence. (E) Expression of pluripotency-associated markers TRA1-60, SSEA4, and SSEA3 by FACS analysis. The upper panel shows the staining using the corresponding irrelevant isotype-matched antibody. (F) In vitro differentiation study by EB formation. EBs were grown for 21 days and induction of genes representative of the three germ layers were analyzed by RT-PCR. Parental ADSCs (G15) were used as negative control. (G) In vivo differentiation test by teratoma formation assay. The pictures show hematoxylin/eosin staining (H&E) with representative tissues from the three germ layers. Pseudostratified epithelium similar to trachea (left), cartilage tissue (middle), and glandular tissues (right).
Pmxs Gw, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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kcnq2 transcript 5 pcdna3.1  (GenScript corporation)
90
GenScript corporation kcnq2 transcript 5 pcdna3.1
Fig. 1. Characterization of G15.AO hiPSC line. (A) G15.AO hiPSC line displays a typical round shape colony morphology with small, tightly packed cells (left panel). Alkaline phosphatase enzymatic activity (Right panel). (B) Endogenous pluripotency-associated markers NANOG, LIN28a, REX1, OCT4, SOX2, <t>KLF4,</t> and cMYC were confirmed by qPCR. MSU hiPSC line and pa- rental ADSCs (G15) were used as positive and negative controls, respectively. (C) Silencing of exogenous reprogramming factors was confirmed by qPCR after passage 15. (D) Expression of pluripotency-associated markers NANOG, OCT4, SOX2, and TRA1-81 at protein level by immunofluorescence. (E) Expression of pluripotency-associated markers TRA1-60, SSEA4, and SSEA3 by FACS analysis. The upper panel shows the staining using the corresponding irrelevant isotype-matched antibody. (F) In vitro differentiation study by EB formation. EBs were grown for 21 days and induction of genes representative of the three germ layers were analyzed by RT-PCR. Parental ADSCs (G15) were used as negative control. (G) In vivo differentiation test by teratoma formation assay. The pictures show hematoxylin/eosin staining (H&E) with representative tissues from the three germ layers. Pseudostratified epithelium similar to trachea (left), cartilage tissue (middle), and glandular tissues (right).
Kcnq2 Transcript 5 Pcdna3.1, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 1. Characterization of G15.AO hiPSC line. (A) G15.AO hiPSC line displays a typical round shape colony morphology with small, tightly packed cells (left panel). Alkaline phosphatase enzymatic activity (Right panel). (B) Endogenous pluripotency-associated markers NANOG, LIN28a, REX1, OCT4, SOX2, KLF4, and cMYC were confirmed by qPCR. MSU hiPSC line and pa- rental ADSCs (G15) were used as positive and negative controls, respectively. (C) Silencing of exogenous reprogramming factors was confirmed by qPCR after passage 15. (D) Expression of pluripotency-associated markers NANOG, OCT4, SOX2, and TRA1-81 at protein level by immunofluorescence. (E) Expression of pluripotency-associated markers TRA1-60, SSEA4, and SSEA3 by FACS analysis. The upper panel shows the staining using the corresponding irrelevant isotype-matched antibody. (F) In vitro differentiation study by EB formation. EBs were grown for 21 days and induction of genes representative of the three germ layers were analyzed by RT-PCR. Parental ADSCs (G15) were used as negative control. (G) In vivo differentiation test by teratoma formation assay. The pictures show hematoxylin/eosin staining (H&E) with representative tissues from the three germ layers. Pseudostratified epithelium similar to trachea (left), cartilage tissue (middle), and glandular tissues (right).

Journal: Stem cell research

Article Title: Generation and characterization of human iPSC line generated from mesenchymal stem cells derived from adipose tissue.

doi: 10.1016/j.scr.2015.12.002

Figure Lengend Snippet: Fig. 1. Characterization of G15.AO hiPSC line. (A) G15.AO hiPSC line displays a typical round shape colony morphology with small, tightly packed cells (left panel). Alkaline phosphatase enzymatic activity (Right panel). (B) Endogenous pluripotency-associated markers NANOG, LIN28a, REX1, OCT4, SOX2, KLF4, and cMYC were confirmed by qPCR. MSU hiPSC line and pa- rental ADSCs (G15) were used as positive and negative controls, respectively. (C) Silencing of exogenous reprogramming factors was confirmed by qPCR after passage 15. (D) Expression of pluripotency-associated markers NANOG, OCT4, SOX2, and TRA1-81 at protein level by immunofluorescence. (E) Expression of pluripotency-associated markers TRA1-60, SSEA4, and SSEA3 by FACS analysis. The upper panel shows the staining using the corresponding irrelevant isotype-matched antibody. (F) In vitro differentiation study by EB formation. EBs were grown for 21 days and induction of genes representative of the three germ layers were analyzed by RT-PCR. Parental ADSCs (G15) were used as negative control. (G) In vivo differentiation test by teratoma formation assay. The pictures show hematoxylin/eosin staining (H&E) with representative tissues from the three germ layers. Pseudostratified epithelium similar to trachea (left), cartilage tissue (middle), and glandular tissues (right).

Article Snippet: To induce cell reprogramming, isolated ADSCs were infected with VSVG-coated MMLV retroviral vectors coding for the human reprogramming factors OCT4, SOX2, c-MYC, and KLF4 (Addgene plasmids 17217, 17218, 17219, and 17220) as described (Takahashi et al. 2007).

Techniques: Activity Assay, Expressing, Staining, In Vitro, Reverse Transcription Polymerase Chain Reaction, Negative Control, In Vivo, Teratoma Formation Assay